King Saud University
  Help (new window)

تحميل الدليل التدريبي

أسئلة شائعة

PHC 523: Analytical Separation Methods



            The course is designed to present different types of liquid chromatography with emphasis on High Performance Liquid Chromatography (HPLC) and recent advances in that area. Furthermore application of HPLC in the quantitation of pharmaceutical compounds is highlighted.


Course Description                                                                       No. of Lectures

A. Introduction


     a) General review of chromatographic separation techniques, including liquid-       3

solid (adsorption), liquid-liquid (partition), ion exchange,

and gel filtration chromatography techniques. Revision of theory

 related to practice of LC such as retention, column efficiency,

selectivity and resolution will also be included.


     b) Ion-pair chromatography                                                                         2

                                i)              General consideration

                                ii)             Role of the counter-ion

                                iii)            Solvent polarity

                                iv)           pH effects

                                v)            Applications


B. Gradient elution

        a)            Introduction                                                                            2

        b)            Design of optimum solvent gradients

        c)            Effect of varying N and a on gradient elution

        d)            Flow and temperature

        e)             Applications


C. Chemical derivatization in liquid chromatography

        a)            Pre-separation techniques

        b)            Post-separation techniques

        c)            Application of UV-visible dericatization

                       in pharmaceutical analysis

        d)            Application of fluorometric derivatization

                       in drugs and pharmaceuticals

        e)             Derivatization and mass spectrometry


D. Microscale HPLC


        a)            Characteristic of microscale HPLC                                               3

        b)            Classification of microscale HPLC

        c)             Fundamental factors in microscale HPLC

                                i)              Peak volume

                                ii)             Maximum peak concentration

                                iii)            Flow rate

        d)            Instrumental requirements for                                                    2

                                microscale HPLC

                                i)              Microscale column

                                ii)             Injection cell volume

                                iii)            Detector cell volume

                                iv)           Connecting tubing

        e)            Microcolumn HPLC-Mass spectrometry                                      2

        f)             Microcolumn HPLC-IR spectrometry

        g)            Application of microscale HPLC


E. Chiral separation in liquid chromatography                                                    4


        a)            Introduction

        b)            Resolution of enantiomers

        c)            Chiral separation of solutes of TLC and HPLC

        d)            Applications


F.High performance thin layer chromatography (HPTLC)                                      2


        a)            Introduction

        b)            Sample preparation and sample application

        c)            Mobile phase selection

        d)            Development techniques for HPTLC

        e)            Detection

        f)            Applications


G. Flash Chromatography                                                                              1


H. Electrophoresis and its application                                                              1


   Two Exams                                                                                             2

                                                                                                           Total     28


King   Saud University. All rights reserved, 2007 | Disclaimer | CiteSeerx