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Indian J Exp Biol. 2007 Oct;45(10):877-89.

Effect of Curcuma longa or parziquantel on Schistosoma mansoni infected mice liver-histological and histochemical study.

El-Banhawey MA, Ashry MA, *El-Ansary AK, Aly SA.

Department of Experimental Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt.*King Saud University, Science college, Biochemistry Department, P.O Box 22452-Zip code 11495.KSA.

Effect of drug praziquantel (PZQ) and C. longa extract on S. mansoni infected mice is reported. The level of glycogen, alkaline and acid phosphatases (ALP and ACP respectively), and body weight, liver weight and liver weight/body weight ratio were studied in mice infected with S. mansoni. ALP level was increased after infection. C. longa treated mice showed marked reduction in ALP level more than after PZQ-treatment. C. longa enhanced the concentration of glycogen after being reduced by infection, while PZQ-treatment revealed more reduction. C. longa caused enhancement in body weight while PZQ treatment had no effect. The formation of granuloma around schistosome eggs in the liver produced inflammation. C. longa extract and PZQ were effective in reducing granuloma size in infected mice.

Indian J Exp Biol. 2007 Sep;45(9):791-801

Antischistosomal and liver protective effects of Curcuma longa extract in Schistosoma mansoni infected mice.

El-Ansary AK, Ahmed SA, Aly SA.

Medicinal Chemistry Department, National Research Centre, Dokki, Cairo, Egypt. King Saud University, Science college, Biochemistry Department, P.O Box 22452-Zip code 11495.KSA.

With a view to clarify the induction of the "Crabtree consequence" in liver cells of S. mansoni infected mice, the curative effect of oil extract of C. longa was tested and compared to praziquantel (PZQ) the effective drug against all schistosome species occurring in man. Protein, glucose, glucose-6-phopsphatase, AMP-deaminase, adensoine deaminase, urea concentration, pyravate kinase (PK), phosphoenol pyruvate carboxykinase (PEPCK) and PK/PEPCK ratio were estimated. In addition, worm burden and ova count in mice infected with S. mansoni were elucidated. The result showed that C. longa normalized the concentration of protein, glucose, AMP-deaminase and adenosine deaminase, which were changed by infection. Moreover, it lowered pyruvate kinase level, while PZQ-treatment induced more elevation of this enzyme. PZQ was more effective in lowering worm burden while C. longa extract was more potent in reducing egg count.

J Egypt Soc Parasitol. 2007 Apr;37(1):39-50.

Effect of sublethal concentration of Solanum nigrum on transaminases and lactate dehydrogenase of Biomphalria arabica, in Saudi Arabia.

El-Ansary AK, Al Daihan SK.

Department of Biochemistry, College of Science, King Saud University, P.O Box 22452, Riyadh 11495, Saudi Arabia.

Schistosomes have a complex lifecycle with freshwater intermediate host snails. The snail host represents the weakest point in the lifecycle of parasite. Biomphalaria arabica is intermediate host for Schistosoma mansoni in Saudi Arabia. In this work, aspartate and alanine aminotransferases (AST and ALT) and lactate dehydrogenase (LDH) were measured in the tissue homogenate and haemolymph of B. arabica. Besides, the effect of sublethal concentrations (LC25) of dry powdered Solanum nigrum leaf was tested as plant molluscicide against B. arabica. The studied enzymes were altered in molluscicide-treated snails compared to control. AST and ALT were slightly affected but LDH was the most significantly altered enzyme. The role of the biochemical manipulation in affecting host-parasite relationship was discussed.

 

Med Sci Monit. 2006 Dec;12(12):RA282-292. Epub 2006 Nov 23.

Important aspects of Biomphalaria snail-schistosome interactions as targets for antischistosome drug.

El-Ansary A, Al-Daihan S.

Department of Biochemistry, King Saud University, Riyadh, Saudi Arabia.

The Biomphalaria species are freshwater snails which have a wide distribution and are significant both medically and economically as intermediate hosts for the schistosome parasite, a digenetic trematode causing schistosomiasis, a disease that infects 200 million people, and domestic animals throughout the tropics. The host-parasite relationship is, in principle, a powerful determinant of the biology of infection and disease. Research on snail-schistosome interactions has the potential for making an important contribution to the study of co-evolution or reciprocal adaptation. The association between Biomphalaria and Schistosoma mansoni could well be an excellent model for studies aimed at elucidating some aspects of the compatibility or resistance of this species to schistosomes. Snail hosts and schistosomes appear to have effects on each other's phenotype and genotype. The objective of this review is to clarify the nature of the relationship between schistosome parasites and their freshwater snail hosts. Aspects of snail-schistosome interactions will be traced in relation to behavioral (growth, reproduction, locomotion), immunological, and biochemical changes induced in the host's tissues by the developing intramolluscan stages of the parasite. This may help to identify biochemical or genetic targets for drug design. Manipulation of the intermediate host through these targets could break the cycle of human and snail infection by schistosomes.

Med Sci Monit. 2005 Mar;11(3):RA94-103.

Stage-specifically expressed schistosome proteins as potential chemotherapeutic targets.

El-Ansary A, Al-Daihan S.

Biochemistry Department, King Saud University, Riyadh, Saudi Arabia. aelansarynrc50@hotmail.com

Digenetic trematodes have evolved behavioral adaptations to their complex life cycles. They have their own strategies to find hosts, locate sites of infection within final and intermediate hosts, escape immune responses, etc. Many of these strategies are highly complex, but they are sometimes quite subtle. While the mechanisms or processes involved in a few parasites are understood, most are not. Schistosoma mansoni parasites inhabit three distinct environments: water, intermediate molluscan hosts, and a definitive vertebrate host. Although targeted chemotherapy and other public health measures are employed to control schistosomiasis and its spread, there is a need for the development of vaccine and new anti-schistosome drugs. Determining how schistosomes interact with different environments may be one mechanism by which suitable vaccine or chemotherapeutic drugs could be developed. Genes expressed in a stage-specific manner may help us to understand the molecular events controlling the complex life cycle of schistosomes. Various proteins are expressed during the life cycle of schistosomes which are essential for its miracidial infection of molluscan hosts, cercarial penetration of vertebrate skin, evasion of the immune responses of both hosts, and other biological activities needed by schistosomes to complete their complex life cycle. Identifying these stage-specific proteins may uncover hidden aspects of parasite biology and provide useful leads for the development of novel intervention strategies.

J Egypt Soc Parasitol. 2003 Dec;33(3):777-94.

On the pathogenicity of attenuated Schistosoma mansoni cercariae released from metabolically disturbed Biomphalaria alexandrina.

El-Ansary A, Mohamed AM, Mahmoud SS, El-Bardicy S.

Department of Medicinal Chemistry, National Research Centre, Dokki, Cairo, Egypt. Aelansarynrc50@hotmail.com

Biomphalaria alexandrina were treated with sublethal concentrations (LC10) of dry powdered leaves Solanum nigrum or whole dry Ambrosia maritima. The two plants affected the development of schistosome parasite within snails by disturbing the glycolytic flux, the most important metabolic pathway for schistosome-infected snails. Attenuated cercariae released from treated snails were used to infect male albino mice to evaluate their pathogenicity compared to control cercariae shed from untreated snails. The mean number of worms established declined from about 70 +/- 17.6 worms/mouse infected with control cercariae to 23.4 +/- 20.33 and 14.18 +/- 10.06 worms/mouse infected with S. nigrum and A. maritima-attenuated cercariae respectively. Most males and females detected in these animals measured 0.4-0.6 and 0.6-1.2 mm respectively compared to 1.2-1.4 and 1.4-1.7 mm in males and females released from mice infected with normal cercariae. Egg count in the liver and intestine of mice infected with attenuated cercariae was remarkably lower showing reduced fecundity of worms developed from attenuated cercariae. Number and size of granulomatous reactions showed remarkable reduction in attenuated cercariae-infected mice. Biochemical analyses for pathogenicity achieved with attenuated cercariae showed that while serum aspartate and alanine aminotransferases (AST &ALT) were more or less similar, depleted glycogen and elevated lipid peroxides were normalized when compared to those infected with normal cercariae.

 

 

Comp Biochem Physiol B Biochem Mol Biol. 2003 Oct;136(2):227-43.

Biochemical and immunological adaptation in schistosome parasitism.

El-Ansary A.

Biochemistry Department, King Saud University, P.O. Box 22452, Riyadah, Saudi Arabia. aelansarynrc50@hotmail.com

The objective of this review is to clarify aspects of immunological and biochemical adaptations of schistosomes to their intermediate and final mammalian hosts. Adaptations to the mammalian hosts are traced in relation to cercarial penetration of mammalian skin, glucose transport and metabolism. The unusual ability of schistosome surface membrane to escape immune recognition and damage are reviewed. Moreover, the behavioural changes induced in the intermediate hosts by schistosomes are considered. The evolutionary adaptation to molluscan hosts aims to increase the probability of transmission of the parasite into its mammalian host. This review inspires more hope for further design of anti-schistosome drugs through disturbing aspects of biochemical and immunological adaptations in schistosome parasitism.

Comp Biochem Physiol B Biochem Mol Biol. 2002 Mar;131(3):535-42.

Effects of carnosine on bilharzial infestation in hamsters: biochemical and histochemical studies.

Soliman KM, Abdel Aziz M, Nassar YH, Abdel-Sattar S, El-Ansary A.

Department of Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt. solimank2002@yahoo.com

We tested the ability of carnosine to improve some liver disorders induced by Schistosoma mansoni parasite in hamsters (Mesocricetus auratus). Results indicate that parasitic infestation induced elevation in serum alkaline phosphatase, gamma-glutamyl transferase, aspartate aminotransferase and procollagen III peptide as a marker of liver fibrosis. Administration of carnosine (10 mg/day) for 15 days either concurrent with infection, 2 and 4 weeks post-infestation was effective in reducing differential worm burden. It was also effective in renormalizing blood glucose level depending on the time course. The most evident effect of carnosine was on serum procollagen III peptide level, which was lowered in infested groups treated with carnosine. Histopathological studies confirmed the potential use of carnosine for intervention in schistosomiasis.

Cutaneous and Ocular Toxicology, Volume 21, Issue 4 December 2002 , pages 307 - 327

THE PROTECTIVE POTENCY OF VITAMINS E AND C IN METHANOL-INDUCED OXIDATIVE STRESS AND RETINOTOXICITY

Authors: Nagla K. El-Sayed a;  Khadiga M. Gaafar aAfaf K. El-Ansary b; Amal I. Osman a
Affiliations:   a Zoology Department, Faculty of Science, Cairo University, Egypt
b Medicinal Chemistry Department, National Research Center, Egypt
 

 

The protective effects of vitamins E and C against methanol-induced free radical production with its subsequent tissue injury in liver and retina of male albino rats were assessed. The rats were divided into four groups: (1) control, (2) antioxidant (Ao) control group receiving 5 mg of each vitamin, E and C, (3) daily ip-injected methanol (2 mL/kg b.wt.) group, killed after three and six doses, and (4) Ao/methanol group administered the vitamins 3 weeks prior to and along with methanol injection and killed as the latter group [Sharpe et al. Methanol Optic Neuropathy: A Histopathological Study. Neurology 1982, 32 (10), 1093-1100].

Methyl alcohol drastically altered the Ao defense system and energy status of rat liver, where highly significant depletion of glutathione levels and inhibition of superoxide dismutase activity, concurrent with significant increase in thiobarbituric acid reactive substances indicating marked elevation in lipid peroxidation. These effects were reversed when the vitamins were administered denoting their role in promoting the Ao defense system. Furthermore, they also increased the methanol-induced depletion in adenylate energy charge, phosphate potential, and ATP values. The amelioration in the energy status as a result of vitamins E and C supplementation suggests that their role as Aos is effective in relieving the impaired oxidative phosphorylation in order to increase the energy demand under physiologically stressful conditions.

Histopathological and ultrastructural results of rat retina confirmed the protective effect of Ao vitamins. As compared to the methanol-intoxicated group, the protected group showed preservation of the membranous structures of the retinal cells, especially mitochondria that assumed their normal shape. This may be attributed to the inhibition of free radical production and lipid peroxidation and subsequently minimum degree of tissue damage. Amelioration of mitochondrial structure reflected the improvement of impaired oxidative phosphorylation of intoxicated animals with an approximately normal level of energy demand. This suggests that Ao vitamins may alter the retinotoxic effects of methanol by promoting the internal Ao defense system and preserving the energy status of the animal.
Keywords: Retina; Retinotoxicity; Methanol; Oxidative stress; Vitamin E; Vitamin C; Antioxidant; Energy charge; Lipid peroxidation; Glutathione
view references (77)

  

J Egypt Soc Parasitol. 2001 Dec;31(3):657-69.

In vivo, attenuation of schistosome cercarial development and disturbance of egg laying capacity in Biomphalaria alexandrina using sublethal concentrations of plant molluscicides.

el-Ansary A, Sammour EM, Soliman MS, Gawish FA.

Department of Medicinal Chemistry, National Research Centre, Dokki, Giza, Egypt. aelansarynrc50@hotmail.com

The dry powdered of Sinapis arvensis, Thymelaea hirsuta, Callistemon lanceolatus and Peganum harmala showed molluscicidal activity against Biomphalaria alexandrina, specific intermediate hosts to Schistosoma mansoni. Effect of LC25 of dry powdered plant molluscicides on hexokinase (HK), glucose phosphate isomerase (GPI), AMP deaminase, adenosine deaminase and phenol oxidase (PO) of B. alexandrina was traced. C. lanceolatus showed the highest molluscicidal activity as it has the lowest LC50 compared to S. arvensis, T. hirsuta, and P. harmala. LC25 of the latter three plants resulted in more significant inhibition of HK, GPI, AMP-deaminase and PO than C. lanceolatus. Treatment of snails with LC10 of these plants markedly affected compatibility of B. alexandrina to S. mansoni infection. Significant decrease in cercarial production recorded in snails treated with sublethal concentrations of S. arvensis, T. hirsuta, and P. harmala. Remarkable impairment of the egg laying capacity of molluscicide-treated snails was also recorded. Correlation between activity levels of HK, GPI and AMP deaminase and compatibility to parasitic infection and role of PO in the egglaying capacity of these snail species were discussed.

Comp Biochem Physiol B Biochem Mol Biol. 2001 May;129(1):157-64.

Effect of carnosine administration on metabolic parameters in bilharzia-infected hamsters.

Soliman K, El-Ansary A, Mohamed AM.

Biochemistry Department, Faculty of Medicine, Cairo University, Cairo, Egypt. solimank2002@yahoo.com

Carnosine is a naturally occurring dipeptide (beta-alanyl-L-histidine) found in muscles, brain and other tissues. This study was designed to test the ability of carnosine to offset metabolic disturbances induced by Schistosoma mansoni parasitism. Results indicate that parasitic infection caused elevation of liver weight/body weight in S. mansoni-infected hamsters, induced lipid peroxidation and reduced glycogen levels. Moreover, adenylate energy charge (AEC) and ATP/ADP and ATP/AMP concentration ratios were markedly lower in infected hamsters. Administration of carnosine (10 mg/day) for 15 days concurrent with infection effectively reduced worm burden and egg count. Administration of carnosine 2 and 4 weeks post-exposure only partially ameliorated the S. mansoni effects on metabolism. Carnosine treatment also normalized most of the parameters measured, including glycogen repletion, the antioxidant status and AEC. These finding support the use of carnosine for possible intervention in schistosomiasis.

J Egypt Soc Parasitol. 2000 Dec;30(3):809-19.

Sublethal concentration of Ambrosia maritima(Damsissa) affecting compatibility of Biomphalaria alexandrina snails to infection with Schistosoma mansoni through disturbing the glycolytic pathway.

El-Ansary A, el-Bardicy S, Soliman MS, Zayed N.

Department of Medicinal Chemistry, National Research Centre, Dokki, Giza, Egypt.

High glycolytic flux as an emergency pathway for generating ATP was recorded as the most important metabolic pathway required for the success of Biomphalaria-Schistosome sporocyst interaction. Effect of LC25 of dry powdered Ambrosia maritima (Damsissa) as plant molluscicide on hexokinase (HK), pyruvate kinase(PK), glucose phosphate isomerase(GPI) was tested. It resulted in a significant inhibition of the three investigated enzymes. Treatment of snails with LC10 concentrations of A. maritima reduced considerably the infection rate of Biomphalaria alexandrina with Schistosoma mansoni to be 34% compared to an infection rate of 80% in control non-treated snails. Longer prepatent period and remarkable decrease in cercarial production was also recorded in snails treated with the sublethal concentrations of this molluscicide.

J Egypt Soc Parasitol. 2000 Aug;30(2):547-60.

Susceptibility of Biomphalaria alexandrina to infection with Schistosoma mansoni: correlation with the activity of certain glycolytic enzymes.

El-Ansary A, Elham , Sammour M, Mohamed AM.

Department of Medicinal Chemistry, National Research Centre, Dokki, Giza, Egypt. aelansarynrc@hotmail.com

The importance of the glycolytic flux for the success of Biomphalaria-Schistosome sporocyst interaction was acertained in this study. Hexokinase (HK), pyruvate kinase(PK), glucose phosphate isomerase(GPI) and lactate dehydrogenase (LD) as four important glycolytic enzymes were markedly stimulated in trematode infected Biomphalaria alexandrina when measured two weeks post exposure to infection with Schistosoma mansoni miracidia. On the other hand phosphoenolpyruvate carboxy kinase (PEPCK), glucose-6-phosphatase (G6Pase) and fructose 1,6 diphosphatase(FDPase) as three gluconeogenic enzymes were slightly affected which confirm the importance of the glycolytic pathway for schistosome-exposed snails. Effect of LC25 of Solanum nigrum leaves dry powder as plant molluscicide on HK, PK and GPI were tested. Treatment with this plant resulted in a significant inhibition of these three investigated enzymes. LC10 concentrations of S. nigrum reduced considerably the infection rate of B. alexandrina with S. mansoni to be 34% compared to an infection rate of 80% in control, non-treated snails. Longer prepatent period and remarkable decrease in cercarial production was also recorded in snails treated with the sublethal concentrations of the molluscicide. As conclusion, susceptibility of B. alexandrina to infection with the digenetic trematode S. mansoni is correlated to the activity levels of the glycolytic enzymes. Moreover, sublethal and less pollutant concentration of S. nigrum could be recommended to control schistosomiasis by disturbing the intramolluscan environment of the parasite.

J Egypt Soc Parasitol. 2000 Aug; 30(2):455-68.

Effect of carnosine administration on certain metabolic parameters in bilharzial infected hamsters.

Soliman KM, El-Ansary AK, Mohamed AM.

Department of Biochemistry, Faculty of Medicine, Cairo University, Egypt.

This study was designed to test the ability of carnosine to cure the metabolic disturbances induced by Schistosoma mansoni parasite. Results indicate that parasitic infection caused elevation of liver weight/body weight of S. mansoni infected hamsters, induced lipid peroxidation and reduced glycogen level. Moreover, the adenylate energy charge (AEC), ATP/ADP and ATP/AMP concentration ratios were markedly lower in infected hamsters. Administration of carnosine (10 mg/day) for 15 days either concurrent with infection, 2 and 4 weeks post exposure was effective in reducing worm burden and egg count only when given at the time of infection. It was also effective in renormalizing most of the measured parameters confirming the glycogen repletion, the antioxidant and AEC correcting actions of carnosine.

J Egypt Soc Parasitol. 2000 Apr;30(1):125-36.

SDS-PAGE-separated tissue proteins of Biomphalaria alexandrina snails in the presence and absence of Schistosoma mansoni.

El-Ansary A, el-Shaikh K, el-Sherbini M.

Department of Medicinal Chemistry, National Research Centre, Dokki, Giza, Egypt.

SDS-PAGE was used to separate tissue proteins of control and trematode-infected Biomphalaria alexandrina snails. The separated profiles demonstrate the occasional appearance of protein fractions and the remarkable increase of concentration of certain molecular masses in infected snails at one week interval over four weeks post exposure to Schistosoma mansoni. Proteins of molecular masses of 44, 56, 65 and 144 KDa were among these occasionally appeared protein masses. Post exposure to S. mansoni larval infection, a protein mass of 36 KDa was predominant giving a markedely higher absorbance (> 1) compared to control (0.0166). This was identified as lactate dehydrogenase enzyme. Moreover, a protein of 56 KDa mass was identified as Pyruvate kinase. The predicted induction of these two enzymes could be either of host and/or parasitic origin. This study revealed that S. mansoni- B. alexandrina complex has a completely different protein pattern compared to control with very low similarity coefficient "S" value. A correlation between the snail tissue protein or separation patterns and the metabolic redirection of the snail host by the developing sporocyst was discussed.

Cell Mol Biol (Noisy-le-grand). 1993 Jun;39(4):449-54. Links

Kinetic potentials of certain scavenger enzymes in fresh water snails susceptible and non-susceptible to Schistosoma infection.

Nabih I, El Ansary A.

Medicinal Chemistry Laboratory, National Research Center, Dokki, Cairo, Egypt.

The activities of catalase (H2O2-oxidoreductase EC 1.11.1.6)- and glutathione reductase (EC 1.6.4.2) as two important scavenger enzymes, were measured in tissue homogenates of Biomphalaria alexandrina and Bulinus truncatus, the snail vectors of Schistosomiasis. A parallel study was done on Lymnea truncatula snails which are not susceptible to Schistosoma infection. The apparent Michaelis constant (Km) for both anzymes were determined in tissue homogenates of the three studied species. The results obtained showed that both susceptible species have higher affinity to hydrogen peroxide (H2O2) and oxidized glutathione (GSSG) than the non-susceptible one.

Comp Biochem Physiol B. 1992 Apr;101(4):499-508.

Metabolic end-products in parasitic helminths and their intermediate hosts.

Nabih I, El-Ansary A.

Department of Medicinal Chemistry, National Research Center, Cairo, Egypt.

Oxidoreductases which control the metabolic end-products in helminth parasites and their intermediate hosts were reviewed, in a trial to elucidate the respiratory metabolism during host-parasite associations. Special attention was given to Schistosoma parasites and their molluscan hosts.

Cell Mol Biol. 1992 Apr;38(2):131-4.

Succinate-DCPIP and NADH-fumarate oxidoreductases in fresh water snails susceptible and non susceptible to schistosoma infection.

Nabih I, El-Ansary A.

Medicinal Chemistry Laboratory, National Research Centre, Dokki, Cairo, Egypt.

The activities of succinate-DCPIP oxidoreductase (SO) and NADH-fumarate oxidoreductase (FR) were determined in tissue homogenate of Biomphalaria alexandrina and Bulinus truncatus, the snail vectors of Schistosomiasia. A parallel study was done on Lymnea truncatula snails which are not susceptible to Schistosoma infection. The Michaelis constant (Km) and maximum velocities (Vmax) for fumarate reduction and succinate oxidation by the tissue homogenates from the three species were determined. The results obtained showed that both susceptible species are aerobic and lactate is the sole end product of anaerobic glycolysis. Lymnea truncatula snails are facultative anaerobic producing succinate as a major end product in the glycolytic pathway.

Cell Mol Biol. 1991;37(4):385-90.

Variation of transaminases and lactate dehydrogenase in irradiated Biomphalaria alexandrina snails.

Nabih I, El-Ansary A.

Medicinal Chemistry Laboratory, National Research Centre, Dokki, Cairo, Egypt.

Effect of ultraviolet and gamma radiations on the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LD) in Biomphalaria alexandrina snails, the specific intermediate host of schistosomiasis, was investigated. Changes in the electrophoretic pattern of LD in the species under study were also taken as a measured parameter and the effect of gamma-irradiation on the glutathione content in the haemolymph of the snails have been included.

Cell Mol Biol. 1991;37(1):1-7.

Inhibition of lactate dehydrogenase isoenzyme associated with anaerobic respiration in schistosomiasis intermediate host snails.

Nabih I, el Dardiri Z, El-Ansary A.

Medicinal Chemistry Laboratory, National Research Centre, Dokki, Cairo, Egypt.

Lactate dehydrogenase isoenzyme (LD5) which is associated with anaerobic respiration was inhibited to a certain degree in Biomphalaria alexandrina snails, the intermediate host for Schistosoma mansoni. Urea and thiourea were used as inhibitors. The effect of LD5 inhibition on the mortality rate of infected Biomphalaria alexandrina snails and on the susceptibility of the snails to the trematode infection was also studied.

Cell Mol Biol. 1991;37(3):309-14.

Activity of some hydrolytic enzymes in tissue homogenates and haemolymph of fresh water snails, intermediate hosts in schistosomiasis.

Nabih I, El-Ansary A.

Medicinal Chemistry Laboratory, National Research Centre, Cairo, Egypt.

The activities of 5-nucleotidase (Ec.3.1.3.5), alkaline phosphatase (Ec.3.1.3.1), glucose-6-phosphatase (Ec.3.1.3.9), and ribonuclease (Ec.3.1.13) had been measured in tissue homogenate and in haemolymph of Biomphalaria alexandrina, the specific intermediate host for the parasitic disease schistosomiasis, induced by the parasite Schistosoma mansoni.

Cell Mol Biol. 1990;36(4):375-81.

Kinetic properties of two transaminases and lactate dehydrogenase of Biomphalaria alexandrina snails, intermediate hosts of Schistosoma mansoni.

Nabih I, el Dardiri Z, El Ansary A, Ahmed SA.

Medicinal Chemistry Laboratory, National Research Centre, Cairo, Egypt.

Aspartate (AST) and alanine (ALT) aminotransferase together with lactate dehydrogenase (LD) from the tissue homogenate of the Biomphalaria alexandrina snails, were partially characterized by measuring the Michaelis constant (km) and the maximum velocity (Vmax). The isoenzymatic pattern of lactate dehydrogenase was investigated through polyacrylamide gel electrophoresis.

 

 

 

Cell Mol Biol. 1990;36(6):637-42.

Measurement of some selected enzymatic activities in infected Biomphalaria alexandrina snails.

Nabih I, el Dardiri Z, El-Ansary A, Rizk M.

Medicinal Chemistry Laboratory, National Research Centre, Cairo, Egypt.

The activities of aspartate (AST) and alanine (ALT) aminotransferases and that of lactate dehydrogenase (LD) were measured in the homogenate of infected Biomphalaria alexandrina snails, the specific intermediate hosts for the parasite Schistosoma mansoni which is the cause of the disease schistosomiasis. The isoenzymatic pattern of LD was also studied in the infected snails tissue.

Cell Mol Biol. 1989;35(2):181-5.

Selected enzymatic activities in fresh water snails, specific intermediate host for human schistosomiasis.

Nabih I, el-Dardire Z, El-Ansary A.

The activities of aspartate aminotransferase (AST) (EC.2.6.1.1.) I, alanine aminotransferase (ALT) (EC.2.6.1.2) II and lactate dehydrogenase (LD) (EC.1.1.1.27) III have been measured in tissue homogenate and in haemolymph of Biomphalaria alexandrina snails, the specific intermediate host for the human parasitic disease schistosomiasis due to Schistosoma mansoni.

 

 

 

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