INVIVO RAPID MOBILIZATION OF ADIPOSE TISSUE BY LIPID APHERESIS - A NEWLY DEVELOPED TECHNIQUE
N.X. Fang, K. Kostner, A. Dwivedy, M. Mahon, T. Shafey, B. Chain.
University of Queensland, Royal Brisbane Hospital , Brisbane - Australia.
Coronary heart disease (CHD) is multifactorial. There are indications that hypertriglyceridaemia is a factor in CHD. Here, we present a new approach termed "lipid-apheresis" (LA) which causes rapid mobilization of induced deposition of adipose tissue in an avian model. Four groups of 5 roosters were used in this study. Hyperlipidaemia and deposition of adipose tissue were induced in two groups by feeding them a diet high in cholesterol and by injection of oestradiol benzoate. Plasma triglyceride concentration increased by a factor of 50 in the estrogenized birds. The other two groups served as normolipidaemic controls.
LA was applied 5 times during the six weeks experimental period to one group of the normolipidaemic and one group of the hyperlipidaemic birds. LA consisted of the removal of 25% of the blood volume, separation of red blood cells (RBC) and delipidation of plasma by a solvent mixture of Butanol and Diisopropyl ether. The delipidated plasma was mixed with the RBC and reinjected into the birds. Delipidation removed all neutral lipids and approximately 50% of phospholipids. LA immediately reduced plasma triglyceride concentration by approximately 25% . However, the reduction of plasma triglyceride concentration was only transient. Within one hour after LA the plasma triglyceride concentration returned to the original value. Haematological and biochemical parameters were not affected. Postmortem examination indicated mobilization of fat from the abdominal adipose tissues in the LA treated hyperlipidaemic roosters.
These preliminary results indicate that LA caused rapid mobilization of induced adipose tissue in chickens. It has yet to be ascertained that this phenomenon will also be applicable to mammals.