European Atherosclerosis Society
RAPID REGRESSION OF ATHEROSCLEROSIS BY CHOLESTEROL APHERESIS - A NEWLY DEVELOPED TECBNIQUE
B. Cham, K Kostner, T. Shafey, ,N.X. Fang, A. Dwivedy,
University of QUEENSLAND, ROYAL Brisbane HOSPITAL, Brisbane - Australia
Abstract. Diet, drug therapy and more recently, LDL apheresis are beneficial for the treatment of atherosclorosis. However, the therapy periods to produce some benefit with these treatments. are long. Here, we present a new approach termed "cholesterol-apheresis" (CA) which causes rapid regression of induced atherosclerosis in an avian model. Four groups of 5 roosters were used in this study. Hyperlipidaemia and atherosclerosis were induced in two groups by feeding them a diet high in cholesterol and by injection of oestradial benzoate. Plasma cholesterol concentration increased by a factor of 8 and triglyccrides concentration increased by a factor of 50 in the estrogenized birds. The other two groups served as normolipidaemic controls.
CA was applied S times during the six weeks experimental period to one group of the normolipidaemic and one group of the hyperlipidaemic birds. CA consisted of the removal of 25% of the blood volume, separation of red blood cells (RBC) and delipidation of plasma by a solvent mixture of Butanol and Diisopropyl ether. The delipidated plasma was mixed with the RBC and reinjected into the birds. Delipidation removed all neutral lipids and approximately 50% of phospholipids. CA reduced plasma total cholesterol and triglycerides. Increases in plasma lecithin cholesterol acyl transferase activity and cholesterol ester concentration were observed. Furthermore, plasma concentration of apolipoprotein A1 also increased. Haematological and biochemical parameters were not affected. Postmortem examination indicated mobilization of fat from the abdominal adipose tissues. Histological analyses of the aorta revealed rapid regression of atherosclerosis.
These preliminary results indicate that CA caused rapid regression of induced atherosclerosis in chickens. It has yet to be ascertained that this phenomenon will also be applicable to mammals.