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تحميل الدليل التدريبي

أسئلة شائعة




 Laboratory department (third year – second semester)

Course code

Course Title

Actual Hours

Credit Hours





MLT 302






The course is designed to give the students with the basics theoretical understanding and skill in:

1. Preparation of different types of specimens for microscopic examination

2. Enabling the student to know the histology of different types of tissues.




 Safety measures:                                                                                         2hrs                                                

(a) Precautions during cutting sections

(b) Precaution during decalcification

(c) Precaution in handling; flammables, corrosives,

clearing agents, carcinogens, Heavy metals,

fixatives and fresh tissues

Microtomes:                                                                                            2 hours


(b) Types

(c) Uses

Microtome Knives:                                                                                   2 hours

(a) Types

(b) Selection

(c) Material used for sharpening including honing

and polishing.

Section cutting:                                                                                       2 hours

(a) Equipment required

(b) Preparation of paraffin section

(c) Technique for sectioning (cutting)

(d) Tissue thickness and faults during cutting

(e) Floatation and picking up of sections on slides

Section adhesives

Frozen sections:                                                                                    2 hours

(a) Definition

(b) Indications

(c) Methods of freezing the tissue

(d) The use of cryostat

(e) Quenching

(f) Staining

Staining:                                                                                                   8 hours

• Significance of staining

• Types of staining - Natural and Artificial

• Theories of staining (selective, metallic, etc)

• Staining methods (vital, direct, direct & indirect,

regressive, progressive, basic acidic, neutral and

physical dye- mordant, Auxochrome,


• Routine staining:

• Haematoxyllin and Eosin

Special staining:

(a) Peds Prussian Blue

(b) Periodic Acid Schiff

(c) Oil Red 0 or Sudan stain

(d) Ziehl Neelsen

(e) Giemsa stain

(f) Methamine silver

(g) Reticulin stain

(h) Masson trichrome

(i) Brown and Brenn

U) Weigerts Van Gieson Stain

(k) Papanicolaou staining

Immunocytochemistry:                                                                            2 hours

(a) Definition

(b) Fixation

(c) Enzyme digestion method

(d) Immunocytochemical technique

(e) DNA probes and their applications

Mounting media:                                                                                      2 hours

(a) Definition

(b) Characteristics of a good mountant media

(c) Types of Ringing media

(d) Technique for mounting

Exfoliative Cytology:                                                                               4 hours

(a) Definition

(b) Importance of preparation of smears

(c) Collection of specimen for cytological studies

from different parts of the body eg

Gynaecological, Respiratory tract, Body fluids-

Pleural & Ascitic, Genital urinary tract, Breast

and Fine needle aspirate (FNAC).

(d) Examinaton of sex chromatin (Buccal smear)

Specimen collection, receiving and Labeling:                                        2 hours

(a) Type of specimens - Surgical, Gynaecological

smear, Non Gynaecological as body fluids, nipple

discharge, scrape smear from eye, buccal etc, fine

needle aspiration, semen analysis

(b) Labelling of specimens and registration

(c) Rejection of specimen policy

Quality Control:                                                                                        2 hours

(a) Definition

(b) Objective measures

(c) Preventive measures


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