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Guidelines_English_Final
تحميل الدليل التدريبي

أسئلة شائعة


 

 

Department              :           Clinical Laboratory Sciences

Course Number      :           CLS 453

Course Title             :           Medical Genetics

Credit Hours            :           2 + 1 = 3

 

 

 

Course Description:

This course is designed to give a basic understanding of the theoretical and practical aspects of medical genetics. In addition to molecular medical genetics includes cytogenetics, DNA, RNA, mutation, mutation detection technique, PCR, cloning, population genetics and clinical genetics. In the practical applies al the technique used in medical genetics like gel electrophoresis, DNA extraction, PCR and bioinformatics.

 

 

 

CLS 453: Laboratory Schedule:

Weeks

Subjects

 

1.       

Safety and Precautions in pre-PCR, PCR and post-

PCR: avoiding contamination; how to handle DNA

Samples

 

2.       

Buffer and Electrophoresis:

- TAE Stock Buffer

- TBE Stock Buffer

- Agarose Gel

- Polyacrylamide Gel (PAGE)

- Stains

 

3.       

DNA Extraction

RNA Extraction

Tissue Extraction

 

4.       

5.       

. PCR Amplification

Design Primers

Reagents to be used (Kit)

DNA Marker (Ladder)

 

6.       

Bioinformatics:

- Database of Genes

- Gene Bank

- Gene Map

 

7.       

Technique used after PCR

- Mutation Detection

- SSCP

- DHPLC

- Restriction Enzymes

- ARMS

- ASO

- Sequencing

 

8.       

9.       

10.  

11.  

12.  

13.  

 

  

References:

 Margaret W.Thompson, Genetics in Medicine, W B Saunders Co; 5th edition (January 15, 1991).

 

 

 
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