1,2. Safety procedures :
- Minimal safety precautions
- Contaminated equipment / glassware
- Chemical hazards
- Hepatitis, AIDS etc .
- Laboratory safety manual
Specimen handling / processing :
- Master log/ lab. Etc .
- Distribution to work areas
- Test protocol
- Analysis, collection of data
- Storage of specimens
- Limitations on storage
Quality Control :
- Quality control on equipment , reagents , standards etc.
- Quality control procedures followed in the laboratory
- Review previous 3 months Quality control data
(charts, graphs etc.)
- Critique on the procedures followed
Orders, supplies, equipment :
Laboratory administration :
- Technical , bench work
- Space utilization
3,4,5. Special chemistry – Radioimmunoassay
Lecture and tutorial :
For each test , review of clinical significance, principle employed in a test
Procedure, test protocol , results, interpretation of data , and Quality control .
Trouble shooting in test procedure / equipment .
Orientation to RIA laboratory , test protocols, use of radiolabel material
quality control .
RIA of a hormone utilizing the procedure on a protein / steroid hormone
employing a double antibody for separation method. calculation of results
and interpretation of the data .
RIA of a hormone employed solid phase method.
Calculation of results and interpretation of the data .
6,7,8. Special chemistry – Electrophoresis
Lecture tutorial and hospital schedule:
Serum protein Electrophoresis :
Procedure , results and interpretation of results .
Isoenzymes – lactate dehydrogenate – Enzymology localization results and interpretation
Haemoglobinopathies, thalassemias , haemoglobin electrophoresis, results
and interpretation .
and interpretation .
9-14. Routine chemistry
Lecture, tutorial and hospital schedule:
Use of automated and other equipment for routine tests performed on
serum , plasma or urine, to include the following :
Glucose, urea, creatinine , sodium , potassium , chloride, CO2 , uric
acid , calcium, CPK, billirubin , albumin , cholesterol , triglycerides,
alkaline phosphates, amylase, acid phosphates, ALT and AST .