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تحميل الدليل التدريبي

أسئلة شائعة


: Clinical Laboratory Sciences

Course number

: CLS 432

Course title

: Clinical practice in Biochemistry

Credit hours

: 1 + 1 = 2



Course Description :


This course is designed to train students in routine procedures performed in a biochemistry laboratory . Laboratory part of the course will be done under the supervision of a trained technologist in a hospital laboratory . 

After completion of the course students should have a good knowledge of laboratory skills required for a clinical chemistry laboratory to include: overall organization, safety precautions, specimen handling and quality control procedures.  They will be able to perform routine and special chemistry test in serum or urine using manual, semi automated or automated equipment .



CLS 432: Laboratory Schedule:

Weeks                                   Subjects


1,2.                                 Safety procedures :

-         Minimal safety precautions

-         Contaminated equipment / glassware

-         Flammables

-         Chemical hazards

-         Radioisotopes

-         Hepatitis, AIDS etc .

-         Laboratory safety manual


                                     Specimen handling / processing : 

-         Receiving 

-         Master log/ lab. Etc . 

-         Distribution to work areas 

-         Test protocol 

-         Analysis, collection of data 

-         Storage of specimens 

-         Limitations on storage


                                   Quality Control : 

-         Quality control on equipment , reagents , standards etc. 

-         Quality control procedures followed in the laboratory 

-         Review previous 3 months Quality control data

                                      (charts,  graphs etc.) 

-         Critique on the procedures followed 

-         Suggestions


                                  Orders, supplies, equipment : 

-         Inventory

-         Orders

-         Supply


                                   Laboratory administration : 

-         Administrative

-         Technical , bench work

-         Space utilization


3,4,5.                        Special chemistry – Radioimmunoassay


Lecture and tutorial :

For each test , review of clinical significance, principle employed in a test

Procedure, test protocol , results, interpretation of data , and Quality              control  .

Trouble shooting in test procedure / equipment .  

Orientation to RIA laboratory , test protocols, use of radiolabel material

quality control .  

RIA of a hormone utilizing the procedure on a protein / steroid hormone

employing a double antibody for separation method. calculation of results

and interpretation of the data .  

RIA of a hormone employed solid phase method.

Calculation of results and interpretation of the data .


6,7,8.                     Special chemistry – Electrophoresis

Lecture tutorial and hospital schedule:

Serum protein Electrophoresis :

Procedure , results and interpretation of results .

 Isoenzymes – lactate dehydrogenate – Enzymology localization results and interpretation  

Haemoglobin :

Haemoglobinopathies, thalassemias , haemoglobin electrophoresis, results

and interpretation .  


and interpretation .  


9-14.                      Routine chemistry


Lecture, tutorial and hospital schedule:


Use of automated and other equipment for routine tests performed on

serum , plasma or urine, to include the following :      

Glucose, urea, creatinine , sodium , potassium , chloride, CO2 , uric

acid , calcium, CPK, billirubin , albumin , cholesterol , triglycerides,

alkaline phosphates, amylase, acid phosphates, ALT and AST .   




1.  Burtis and Ashwood, Teitz fundamentals of Clinical Chemistry, by Saunders.


2.  Bishop, Fody and Choeff, Clinical chemistry: principles, procedures and correlations, by Lippincott.

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