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BCH 232

 Physical Biochemistry


 (3+0) credit-hours


 8:00 - 8:50 (1,3,5)
 Class Room  Bil- 8 3 686

Syllabus and Books -click-


COURSE OVERVIEW: The course objective is to introduce and develop physical concepts for the biochemist by using theories of physical chemistry (rather than pure mathematical formulations) to exemplify biochemically relevant phenomena. The desired outcome is for students to gain a basic understanding of how solution dynamics, hydrodynamics, and spectroscopy….etc can be applied to biochemical problems. 

ATTENDANCE AND PARTICIPATION:  Attendance at all of the class sessions is mandatory unless you are sick.

HOMEWORK: Questions and problems will be given as homework regularly: they are designed to encourage your regular reading of the material and completing them will help you prepare for the exams. 

PERFORMANCE EVALUATON: Grades will be determined by two 50 minute in-class exams, as well as by class participation and three hours final exam. The questions on exams will consist of short answers, multiple-choices, correct statements and assay questions. Questions about exam scores or requests for re-grading must be submitted in writing to the course faculty within one week after any given exam is returned.

   1st    Mid  Exam        24/ 11/ 1431  25   %

    2nd Mid   Exam      14/ 01/ 1432

 25   %
           Final Exam  50   %
 Homework\class Quizzes (Bonus)   5-10 %

MAKE UP EXAMS: You should contact the fuculty either before the exam or in a timeframe after the exam that is consistent with the reason for missing the exam. Makeup exam is at the discretion of the faculty, and may include assay format 30-75%. 

The goals of this course are to answer these questions; how you design a research work, how the bio-kits in research and clinical labs work and how the equipments is built in. 

How to design a research experiment:

Step 1: Identify an important research problem.

Step 2: Clearly define the research objective.

Step 3: Design a study that can address the problem and meet the objective.

Step 4: Select an appropriate analytical method and suitable equipments.

Step 5: Acquisition analysis of samples

Step 6: Data analysis and classifier development

Step 7: Validation and ongoing evaluation of your experiment

Step 8: Publication, patents or new routine testing

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Immunohistochemistry          ELISA         Gel Electrophoresis



 BCH 232 -Lectures

1-1-Membrane Filtration and Dialysis (Nitrocellulose, Glass and Polycarbonat Filters).ppt1-1-Membrane Filtration and Dialysis (Nitrocellulose, Glass and Polycarbonat Filters)د. جيهان جاويش الحسيني
1-2 Membrane Filtration and Dialysis (Equilibrium, Reverse, Modified and Glass fiber Dialysis).ppt1-2 Membrane Filtration and Dialysis (Equilibrium, Reverse, Modified and Glass fiber Dialysis)د. جيهان جاويش الحسيني
2-Hydrodynamic Methods (Sedimentation, Centrifugation and Ultracentrifugation.ppt2-Hydrodynamic Methods (Sedimentation, Centrifugation and Ultracentrifugationد. جيهان جاويش الحسيني
3-1 Electrophoresis and its types (Moving Boundary Electrophoresis, Zone electrophoresis and Gel Electrophoresis.ppt3-1 Electrophoresis and its types (Moving Boundary Electrophoresis, Zone electrophoresis and Gel Electrophoresisد. جيهان جاويش الحسيني
3-2 Electrophoresis (SDS-PAGE  and IEF-PAGE).ppt3-2 Electrophoresis (SDS-PAGE and IEF-PAGE)د. جيهان جاويش الحسيني
4-1- Chromatography (Principles and Classifications).ppt4-1- Chromatography (Principles and Classifications)د. جيهان جاويش الحسيني
4-2-Chromatography (Paper Chromatography and TLC).ppt4-2-Chromatography (Paper Chromatography and TLC)د. جيهان جاويش الحسيني
4-3-Gel Chromatography.ppt4-3-Gel Chromatographyد. جيهان جاويش الحسيني
4-4-Gas Liquid Chromatography.ppt4-4-Gas Liquid Chromatographyد. جيهان جاويش الحسيني
4-5-High Performance Liquid Chromatography.ppt4-5-High Performance Liquid Chromatographyد. جيهان جاويش الحسيني
4-6- Ion Exchange Chromatography.ppt4-6- Ion Exchange Chromatographyد. جيهان جاويش الحسيني
5-1 Introduction to Spectroscopy.pptx5-1 Introduction to Spectroscopyد. جيهان جاويش الحسيني
5-2 Spectroscopy (Nature of radiation, Mesurement Process.pptx5-2 Spectroscopy (Nature of radiation, Mesurement Processد. جيهان جاويش الحسيني
5-3 Spectroscopy (Common Types and Radiation Spectroscopy).pptx5-3 Spectroscopy (Common Types and Radiation Spectroscopy)د. جيهان جاويش الحسيني
5-4 Spectroscopy(Intrinsic and Extrinsic Chromophore - FISH Technique).pptx5-4 Spectroscopy(Intrinsic and Extrinsic Chromophore - FISH Technique)د. جيهان جاويش الحسيني
5-5 Spectroscopy (Factors Affecting the Absorption Properties of Chromophore).pptx5-5 Spectroscopy (Factors Affecting the Absorption Properties of Chromophore)د. جيهان جاويش الحسيني
5-6 Beer Lambert law.pptx5-6 Beer Lambert lawد. جيهان جاويش الحسيني
6- Hydrodynamic Focusing Methods and Flowcytometry.ppt6- Hydrodynamic Focusing Methods and Flowcytometryد. جيهان جاويش الحسيني
7- Immunological methods.ppt7- Immunological methodsد. جيهان جاويش الحسيني
8- Radioisotope tichniques.ppt8- Radioisotope tichniquesد. جيهان جاويش الحسيني
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 BCH 232 Exams

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