I- applications of modern NMR techniques:
1- spectral editing by the APT experiment
Basic principle, interpretation, applications in the identification of functional groups of terpenoids, steroids and cannabinoids.
2- homonuclear correlation techniques cosy, inadequate, noesy, basic principle interpretation, applications in the structure elucidation of selected examples of antibiotics, complex alkaloids and macrocyclic lactones.
3- polarization transfer experiments
INEPT, DEPT, DEPTGL, basic principles, interpretation, applications in the characterization of terpens, steroids, carotenoids and vitamins
4- heteronuclear shift correlation techniques, HMQC, HMBC, TOCSY, principle interpretation, applications in the structure elucidation and mechanism of action of selected examples of antibiotics, carbohydrates and sesquiterpenes.
II- Chemical and spectroscopic techniques used for stereochemical assignments.
1- chemical degradation as a means of determining absolute stereochemistry literature examples from the fields of alkaloids and carbohydrates.
2- Optical rotatory dispersion (ORD) and CD.
3- Application of Horeaus and the benzoate rule for the determination of the absolute stereochemistry of terpenes.