1- Apoptosis and the malaria parasite
We have previously demonstrated that many zygotes and ookinetes of the rodent malaria parasite, Plasmodium berghei, undergo apoptosis-like death whilst in the midgut lumen of the mosquito. They also die in this manner in vitro. Many of the markers of eukaryote apoptosis are exhibited and they react with caspase detectors. We are trying to investigating, is the nitric oxide as a signals that induce this apoptosis-like death in vivo and in vitro.
Plasmodium berghei ookinetes showing signs of programmed cell death. A: an ookinete containing a nucleus with condensed chromatin, labelled with acridine orange. B: an ookinete with caspase-like activity in the cytoplasm (stained green using a CaspaTag kit).
Gene Expression Profiling of Human Macrophage Infected With Leishmania major
Macrophages (MØ’s) are one of the first lines of defense that protect our body from pathogenic infection by recognition and removal of foreign micro organisms. However, species of the protozoan parasite Leishmania not only enter MØ’s, but also use them for growth and proliferation. How does Leishmania contribute the function of Macrophage, will be answered in this project by tracing the effect of exposing Leishmania to macrophage gene expression in different times.
THP1 (human monocytic leukemia cells) was induced into Macrophage in vitro by adding PMA (pholbol 12-myristate 13-acetate). THP1 will be exposed to Leishmania major for 30 min, and 24h. Infected MØ’s RNA will be extracted and the messenger RNA is going to be used for Axon arrays technique to detect the changes in the gene expression of the host cell. We expect the results to be of a great value to discover new molecules and/ or pathways important for the survival of Leishmania within MØ’s.
1- Sero-epidemiological Studies of Toxoplasmosis in Chickens Slaughtered in Riyadh Region.
Many of studies have been proved that the prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the exist of T.gondii oocysts in environment because chickens feed from the ground. However the equations ask is Toxoplasmosis prevalence between chickens (in free-ranging chickens and in farms) in the Riyadh Region and the chickens play as intermediate hosts to prevalence Toxplasmosis such as sheep and gotes because humans depend on chickens as a food. Our data shown that the absence of T.gondii in chickens of farms but on other hands, it found in free-ranging of chickens by serological tests (LAT,IHAT,IFAT,ELISA), PCR and histological examination of the brain tissue and the genotype is type 111.