Examination Model CLS453


 1)    Which process of the following takes place first for gene expression inside the cell:

a.     translation

b.    transcription

c.     replication

d.    splicing

e.    regulation


2)   The first part of the gene part of is called:

a.     Promoter

b.    Intron

c.     Restriction site

d.    Exon

e.    Non of the above


3)   In recombinant DNA:

a.     A DNA molecule created in-vitro to join different pieces of DNA together

b.    Only an identical pieces of DNA can be ligated together

c.     Both DNA polymerase and DNA ligase are used

d.    Alteration in the sequence can not be created

e.     b and d


4)   Sickle cell anemia is caused by:

a.     non-sense mutation in the protein coding region of the Beta-globin gene.

b.    missense mutation in the protein coding region of the Beta-globin gene.

c.     deletion of the Beta-globin gene promoter.

d.    overexpression of the Beta-globin gene.

e.    overexpression of the alpha-globin gene.


5)   In the PCR reaction, the primers are used to:

a.     synthesize a DNA molecules

b.    lead the DNA polymerase for DNA synthesis

c.     supply the template sequence with the dNTPs

d.    stop the PCR at the end of the cycles

e.    separate the two DNA strands at the beginning


6)   The following techniques are used to scan a certain gene for unknown mutation, except:

a.     Conformation sensitive gel electrophoresis

b.    Direct DNA sequencing

c.     Chemical cleavage mismatch

d.    Single-stranded conformational polymorphism

e.    Restriction enzyme digestion


7)   The transfer of the genetic information from DNA to RNA protein is called:

a.     Reading frame

b.    Gene cloning

c.     Translation

d.    Central dogma

e.    None of the above


8)   The restriction enzymes are:

a.     Naturally found in bacteria

b.    Used to cut a DNA fragment at a particular sequence of nucleotides

c.     Used in detecting a known mutation

d.    They can cut both a fragment of DNA and a vector

e.    All of the above


9)   A frame shift mutation is:

a.     A mutation which involve the change of one codon by another

b.    A mutation which involve the insertion of one codon

c.     A mutation which involve the deletion of one amino acid

d.    A mutation that will not affect the reading frame

e.    A mutation in which the change of nucleotides are not a multiple of 3


10)                 A point mutation is:

a.     A frame shift mutation

b.    An unstable trirepeat

c.     Missense mutation

d.    Frame shift mutation

e.    All of the above