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تحميل الدليل التدريبي

أسئلة شائعة

474 PHG: Screening, Separation and Analysis of Plant Constituents (2+2)

The course covers the methods and techniques employed for screening medicinal plants for their bioactive constituents. It also discusses recent developments regarding methods of extraction, isolation, purification and spectroscopic identification of plant bioactive constituents not covered elsewhere. Emphasis is given to the chemistry of certain selected classes of these constituents. In addition, the course includes further applications of chromatographic and spectroscopic techniques in the analysis of the isolated compounds. The students will be trained to use spectral data for interpretation of the structures of the compounds that are isolated in the laboratory part of the course.  



No. of lectures


1.1 General concepts
1.2 Medicinal Plants Information Sources


2. Phytochemical Screening

2.1 Methodologies

- Preparation of the plant material.

- Physicochemical tools and techniques employed.

- General extraction procedures including infusion, decoction, maceration, percolation, and continuous extraction.

2.2 Screening for bioactive plant constituents:

2.3 Specific extraction procedures:
procedures used for the isolation of certain classes of medicinal plant constituents.










3. Purification of the individual components of the isolated chemical classes.

3.1 Chemical methods, including derivatization and related technique.

3.3 Further application of chromatographic separation techniques such as adsorption partition, ion-exchange, gel-filtration, chromatotron, GLC and HPLC.






4. Chemistry and spectroscopic analysis of the isolated compounds.

4.1 Characterization of the active constituents by physical and chemical methods, including

a. Determination of physical parameters.

b. Response to color tests.

c. Chromatographic behavior.

d. Compliance with pharmacopoeial standards.

4.2 Application of the spectroscopic techniques for the identification of the isolated compounds and interpretation of their spectral data.

a. Application to alkaloids.

b. Application to flavonoids.

c. Application to steroids and steroidal glycosides.

d. Application to terpenes, vitamins and antibiotics.









 Lectures 474

474PHG-10.ppt474PHG-10ابتسام سعد فهد الشدى
474PHG-9.pptx474PHG-9ابتسام سعد فهد الشدى
474PHG-8.pptx474PHG-8ابتسام سعد فهد الشدى
474 PHG-7.pptx474 PHG-7ابتسام سعد فهد الشدى
474 PHG 6.ppt474 PHG 6ابتسام سعد فهد الشدى
474 PHG 5.ppt474 PHG 5ابتسام سعد فهد الشدى
474 PHG 4.ppt474 PHG 4ابتسام سعد فهد الشدى
474PHG 3.ppt474PHG 3ابتسام سعد فهد الشدى
lecture 1 474 PHG.pptxlecture 1 474 PHGابتسام سعد فهد الشدى
lecture 2 474 PHG.pptxlecture 2 474 PHGابتسام سعد فهد الشدى
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