BG-11 Medium (modified)

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BG-11 Medium (modified)

Allen and Stanier 1968, Watanabe et al. 2000

 

This medium has an exceptionally high concentration of nitrate and phosphate; no vitamins are included in the recipe.

First, prepare the Fe citrate stock solution by dissolving citrate and ferric ammonium citrate in 1 liter dH2O. To prepare BG-11 medium, begin with 900 mL of dH2O, add 1 mL of the Fe Citrate solution, and then add the remaining components. Autoclave. Final pH 7.4 after cooling and CO2 equilibration.

 

Component

Stock Solution

Quantity

Molar Concentration in Final Medium

Fe Citrate solution

 

1 mL

 

Citric acid

6.0 g L-1 dH2O

---

3.12 x 10-5 M

Ferric ammonium citrate

6.0 g L-1 dH2O

---

---

NaNO3

---

1.5 g

1.76 x 10-2 M

K2HPO4

39.0 g L-1 dH2O

1 mL

2.24 x 10-4 M

MgSO4 • 7H2O

75.0 g L-1 dH2O

1 mL

3.04 x 10-4 M

CaCl2 • 2H2O

27.0 g L-1 dH2O

1 mL

1.84 x 10-4 M

Na2CO3

20.0 g L-1 dH2O

1 mL

1.89 x 10-4 M

Na2SiO3 • 9H2O

58.0 g L-1 dH2O

1 mL

2.04 x 10-4 M

trace metals solution

(see recipe below)

1 mL

---

 

Trace Metals Solution

To prepare, begin with 900 mL of dH2O and dissolve the EDTA.  Next, dissolve each compound and bring  the final volume to 1 liter. Autoclave.

Component

Stock Solution

Quantity

Molar Concentration in Final Medium

MgNa2EDTA • 3H2O

---

1.000 g

2.26 x 10-6 M

H3BO3

---

2.860 g

4.63 x 10-5 M

MgSO4 • 7H2O

---

2.500 g

1.01 x 10-5 M

ZnSO4 • 7H2O

---

0.220 g

7.65 x 10-7 M

CuSO4 • 5H2O

79.0 g L-1 dH2O

1 mL

3.16 x 10-7 M

Na2MoO4 • 2H2O

21.0 g L-1 dH2O

1 mL

8.68 x 10-8 M

Co(NO3)2 • 6H2O

49.4 g L-1 dH2O

1 mL

1.70 x 10-7 M

 

Allen, M.M. & Stanier, R.Y. 1968. Growth and division of some unicellular blue-green algae. J. Gen. Microbiol. 51: 199-202.

Hughes, E.O., Gorham, P.R. & Zehnder, A. 1958. Toxicity of a unialgal culture of Microcystis aeruginosa. Can. J. Microbiol. 4: 225-36.

Watanabe, M.M., Kawachi, M., Hiroki, M. & Kasai, F. 2000. NIES-Collection List of Strains Sixth Edition 2000 Microalgae and Protozoa. Microbial Culture Collections, National Institute for Environmental Studies, Tsukuba, 159 pp.

 



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